Sara Nathalie Edjenguèlè Béboy

Androgenic effects of the aqueous extract of Pycnanthus angolensis (Welw.) Warb. (Myristicaceae) wood in hemicastrated male Wistar rats

Younous Yaya Charfadine, Rodolphe Koularambaye, Sara Nathalie Edjenguèlè Béboy, Rodrigue Fifen, Yannick Sani Jignoua, Paul Désiré Djomeni Dzeufiet et 1 autre(s)

Journal of Drug Delivery and Therapeutics

Background: Androgen deficiency is the most common disorder of reproductive function and can lead to male sexual disorders. Objective: This study was designed to evaluate the androgenic effects of Pycnanthus angolensis (Welw.) Warb in hemicastrated rats. Materials and methods: Forty-two male rats were divided into 6 groups of 7 rats each, including a group of uncastrated rats that received distilled water (10 ml/kg); a group of castrated rats that received 10 ml/kg of distilled water; a group of castrated rats that received testosterone enanthate (3 mg/kg BW) per week intramuscularly; and 3 groups of castrated rats that received 43, 86 and 172 mg/kg of the aqueous extract of Pycnanthus angolensis, respectively. After 14 days of oral treatment, the rats were killed by decapitation. The blood was collected and the androgen-dependent organs were collected for histological sectioning, and biochemical analysis. The tail of the epididymis was used to assess sperm quality. Results: Treatment with the aqueous extract at doses of 43 and 86 mg/kg, significantly improved the sexual behavior of castrated rats, with increases of 25.92% and 22.74% intromissions frequency, and 67.06% and 56.46% mount frequency, compared to those in the castrated rats which did not receive any treatment. The extract also enhanced sperm quality in castrated rats. Both doses also significantly increased serum testosterone levels with rates of 45.07% and 49.00%, respectively; compared to those in the negative control group. Conclusion: In view of the aforesaid results, Pycnanthus angolensis (Welw.) Warb could be considered as a promising natural agent in hypogonadism management. Keywords: Androgen, hypogonadism, castrated male rats, unilateral castration, Pycnanthus angolensis (Welw.) Warb

• #hormonal and reproductive studies
• #sperm and testicular function
• #plant toxicity and pharmacological properties
• #sperm quality

Androgenic Properties and Subchronic Toxicity of the Aqueous Extract of Pycnanthus angolensis (Welw.) Warb. Wood (Myristicaceae)

Rodolphe Koularambaye, Sara Nathalie Edjenguèlè Béboy, Yannick Sani Jignoua, Alfloditte Flore Feudjio, Arnaud Cyrille Choupo, Fabiola Jenny Massah et 2 autre(s)

Journal of Complementary and Alternative Medical Research

Aims: Pycnanthus angolensis (Welw.) Warb. also known as “Etengué” in the Baka language, is a medicinal plant used by the Baka Pygmies of Cameroon to treat erectile dysfunction. This study aimed to evaluate the androgenic activity and subchronic toxicity of the aqueous extract of Pycnanthus angolensis wood. Study Design: Experimental design. Place and Duration of Study: Laboratory of Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, between November 2022 and October 2023. Methodology: Twenty-five male Wistar strain rats were divided into 5 groups of 5 rats each including a positive control group that received testosterone enanthate (5 mg/kg) intramuscularly once a week; a normal control group that received distilled water (10 ml/kg) and three test groups which received 134 and 267 and 533 mg/kg of the aqueous extract of Pycnanthus angolensis daily per os. On the 29th day, the animals were killed, and several biochemical parameters were assessed for androgenic properties. Results: At a dose of 134 mg/kg, the extract significantly increased (p<0.05) the levels of testicular cholesterol, testosterone and serum prostatic acid phosphatase activity compared to those of the normal control. A toxicity study showed that at doses of 134 and 267 mg/kg, the extract did not induce any significant variation (p>0.05) in the creatinine level, but a significant reduction (p<0.05) in of aspartate and alanine aminotransferase activities was recorded. Histopathology revealed destruction of testicular spermatozoa at a dose of 533 mg/kg compared to that of the controls. Conclusion: These results showed that at a dose of 134 mg/kg, the aqueous extract of Pycnanthus angolensis (Welw.) Warb. wood is capable of stimulating the production of androgens and is tolerated by the rat's body. Our results justified the traditional use of this plant for the treatment of male infertility and erectile dysfunction.

• #natural antidiabetic agents studies
• #piperaceae chemical and biological studies
• #biology
• #botany

Anti-reprotoxicity effects of aqueous extract of Terminalia superba Engl. & Diels (Combretaceae) on aluminum chloride exposure of male rats

Yannick Sani Jignoua, Sara Nathalie Edjenguèlè Béboy, Paulin Teko Keumedjio, Marie Louise Coute-Chère Mbog, Paul Fewou Moundipa

Asian Journal of Pharmacy and Pharmacology

Objective: Aluminum chloride can induce toxicity of the reproductive system leading to infertility which is a real public health problem.This study was undertaken to investigate the effect of Engl.& Diels in vivo Terminalia superba aqueous extract on aluminum chloride-induced reprotoxicity in male rats.Thirty male Materials and methods: Wistar rats of 12 weeks each, weighing 160 -180 g were intraperitoneally administered aluminum chloride (20 mg/kg, body weight) for 56 days.The rats were subsequently orally treated for 56 days with Engl.& Diels Terminalia superba aqueous bark extract at doses of 43 and 86 mg/kg and a coadministration of vitamin E (100 mg/kg) and zinc (50 mg/kg).Then, the rats were killed, and blood, testes, epididymis, seminal vesicle and prostate were removed for histological and biochemical analysis.Aluminum chloride caused significant decreases in the sexual performance of the male Results: rats, sperm quality, catalase activity and glutathione levels (P < 0.05).The serum testosterone and fructose levels were also significantly lower in the intoxicated group (P <0.05) than in the normal control group, with decreases of 91.25 % and 8.80 % respectively.In addition, histological lesions were observed in the testes, epididymis and prostate of the intoxicated group.Our results revealed that the aluminum chloride-induced reprotoxicity was reversed by the aqueous extract of Engl.& Diels.Oral administration of an aqueous extract of Terminalia superba Terminalia Conclusion: superba ; Engl.& Diels at the dose of 86 mg/kg, can alleviate the aluminum chloride-induced reprotoxicity in male rats.

• #pharmacological effects of medicinal plants
• #aluminum toxicity and tolerance in plants and animals
• #phytochemicals and medicinal plants
• #combretaceae

Antioxidant Properties and Effects on Nitric Oxide Release of Ethanolic Extracts of Some Selected Cameroonian Medicinal Plants

Sara Nathalie Edjenguèlè Béboy, Paulin Teko Keumedjio, Sylvain Nsangou Pechangou, Yannick Sani Jignoua, Dieudonné Pascal Djamen Chuisseu, Paul Fewou Moundipa

Asian Journal of Research in Medical and Pharmaceutical Sciences

In the Southern region of Cameroon, some medicinal plants are used for the treatment of the male reproductive system disorders; including Palisota ambigua, Rauvolfia macrophylla, Terminalia superba, Pycnanthus angolensis, Pausynistalia yohimbe and Schumanniophyton magnificum. The aim of our study was to investigate the antioxidant properties of the ethanolic extracts of these plants, as well as their phytochemical constituents and their effects on nitric oxide release. Rat peritoneal macrophages were isolated and used to assess in vitro effects of plant extracts on nitric oxide release. Then, antioxidant properties of alcoholic extracts of the plants were screened through 2,2-diphenyl-1-picrylhydrazyle test, total antioxidant capacity test and ferric reducing power test and lipid peroxidation inhibition test. Phytochemical constituents, total phenol and flavonoid contents were also investigated. Results showed that only the extracts of T. superba and P. yohimbe were found to be strong 2,2-diphenyl-1-picrylhydrazyle free radical scavengers with low EC50 (8.40 [8.31, 8.53]; 17.73 [17.67, 17.74] µg of plant extract/mol 2,2-diphenyl-1-picrylhydrazyle). The plants extracts exhibited better inhibitions of the lipid peroxidation in concentration-dependent manner; especially R. macrophylla with an IC50 of 2.38 (2.13, 4.92) µg/mL. No significance difference was observed neither among the total phenolic nor flavonoids contents of the plant extracts. Extract of T. superba exhibited strong antioxidant properties. Though, the extracts of R. macrophylla and P. angolensis and S. magnificum were also able to protect against lipid peroxidation and free radicals. The phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, tannins, coumarins, glycosids in most of the plants extracts. These properties sustain their use for treatment of male infertility and erectile dysfunction.

• #natural antidiabetic agents studies
• #nitric oxide
• #antioxidant
• #traditional medicine

Aqueous extract of Pycnanthus angolensis (Welw.) Warb. (Myristicaceae) alleviates paroxetine-induced erectile dysfunction in male rats

Sara Nathalie Edjenguèlè Béboy, Louise Marie Coûte-Chère Mbog, Yannick Sani Jignoua, Paulin Teko Keumedjio, Patrick Kevin Motto Feune, Paul Fewou Moundipa

The Journal of Phytopharmacology

Background: Erectile dysfunction is the inability to achieve and maintain an adequate erection for sexual performance. Pycnanthus angolensis (Welw.) Warb. is a medicinal plant used by the traditional healers of the Southern region of Cameroon to manage male infertility and especially erectile dysfunction. Aim and objectives: This study aimed at investigating the effects of aqueous extract of Pycnanthus angolensis wood were investigated on paroxetine-induced erectile dysfunction in male rats. Material and methods: Thirty Wistar albino male rats (160-180 g) were randomly distributed into six groups of five animals each. Erectile dysfunction was induced in 5 groups for 21 days by oral administration of paroxetine (10 mg/kg), once a day. After induction, rats were orally treated during 14 days with three doses of plant extract (43, 86 and 172 mg/kg) except 2 groups which were given distilled water and sildenafil (5 mg/kg), respectively. Sexual behaviors were monitored on days 1, 4, 7, and 14 by pairing male rats to receptive females. After 14 days- treatment, the rats were killed by decapitation under ether anesthesia. The reproductive organs were collected for histological and biochemical analysis. Results: Paroxetine induced erectile dysfunction characterized by a significant decrease (p&lt;0.05) in sexual arousal and performance of male rats. Nevertheless, this erectile dysfunction was improved by administration of the aqueous extract of Pycnanthus angolensis at the different doses. The administration of paroxetine significantly (p&lt;0.05) reduced the nitric oxide level by 66.67% when compared to the distilled water-group. After 14 days-treatment, the extract induced significant increases (p&lt;0.05) in the sexual performance parameters (mounts, intromissions and ejaculations frequency) as well as in the sexual arousal of the male rats. Results were markedly pronounced with the plant extract at the doses of 86 and 172 mg/kg. The nitric oxide levels in the erectile dysfunction-induced rats with paroxetine were restored after treatment with the plant extract. The degenerated seminiferous tubules and the low density of spermatozoa observed in the histological sections of the erectile dysfunction-induced rats with paroxetine, were restored after treatments with sildenafil and the aqueous extract at either doses (43, 86 and 172 mg/kg). Conclusions: Aqueous extract of Pycnanthus angolensis (Welw.) Warb alleviated the paroxetine-related erectile dysfunction by enhancing sexual behavior and the production of nitric oxide. In view of the abovementioned results, Pycnanthus angolensis (Welw.) Warb could be considered as an alternative treatment in the erection dysfunction management.

• #attachment and relationship dynamics
• #piperaceae chemical and biological studies
• #maternal mental health during pregnancy and postpartum
• #paroxetine

Isolation, identification of endophytic fungi extracts from Codiaeum variegatum and assessment of their anti-amoebic and anti-inflammatory potential

Espérance Argentine Mbompieze Téfandjam, Sylvain Nsangou Pechangou, Sara Nathalie Edjenguèlè Béboy, Brice Enang, Cynthia Paola Demeni Emoh, Jean Paul Assam Assam et 1 autre(s)

The Journal of Phytopharmacology

Intestinal amoebiasis (IA) remains a global public health concern. It is often confused with an inflammatory bowel disease, and is caused by Entamoeba histolytica. Although it is more widespread in tropical countries, IA is increasingly becoming a subject of concern in so-called developed countries. The limits of conventional therapy and the concern to promote phytotherapy in a sustainable and ecological manner inspired this work, during which the anti-amoebic and anti-inflammatory potential of endophyte fungi extracts isolated from Codiaeum variegatum were evaluated. C. variegatum leaves and bark was harvested at the University of Yaounde 1. Those two fragments undergo surface decontamination then were placed in petri dishes containing PDA (Potato Agar Dextrose) supplemented with amoxicillin. The cultures obtained were then subcultured in SDA (Sabouraud Dextrose Agar) and CZA (CZapek dextrose Agar) media. The resulting growth was observed using an optical microscope with an x40 objective for grouping on the basis of macroscopic and microscopic characteristics. Screening of extracts of these fungi by the Trypan blue counting method on clinical isolates of E. histolytica, made it possible to retain 4 extracts (6, 8, 14, 18) of fungi with anti-amoebic activity. Subsequently, the anti-inflammatory potential of these extracts was evaluated on a primary culture of macrophages activated by Saccharomyces cerevisiae through the inhibition of nitric oxide (NO) production, NADPH oxidase activity, and activation of alkaline phosphatase (ALP). From that culture, 28 endophytic fungi were isolated, 13 belonging to 7 genus (Absidia sp, Alternaria sp, Aspergillus sp, Trametes sp, Trichoderma sp, Fusarium sp and Geotrichum sp). Anti-amoebic tests showed that extract 14 was the most active with an inhibitory concentration 50 (IC50) of 289.87±18.57; 207.73±22.36 and 87.79±12.51µg/mL respectively after 24, 48 and 72 hours of incubation. Chemical screening of the extracts revealed the majority presence of flavonoids in the 4 extracts (6, 8, 14, and 18). The anti-inflammatory tests of these 4 extracts revealed better anti-inflammatory activity in extract 6 (IC50= 95.94 ± 21.07 µg/mL for NO; IC50= 11.78±0.27 µg/mL for NADPH oxidase). Extract 14 had the best effect on the inhibition of NO production (IC50 = 8.56±2.13 µg/mL) and on the inhibition of the growth of trophozoites. Endophytic fungi extract from C. variegatum have demonstrated promising anti-amoebic and anti-inflammatory potential and therefore deserve special attention for the development of natural and ecological alternative treatment.

• #mycobacterium research and diagnosis
• #isolation (microbiology)
• #plant use of endophytic fungi in defense
• #identification (biology)

Protective Effects of Terminalia superba Engl. &amp; Diels (Combretaceae) on Aluminium Chloride-Induced Reproductive Toxicity and Oxidative Stress in Male Wistar Rats

Yannick Sani Jignoua, Sara Nathalie Edjenguèlè Béboy, Sylvain Nsangou Pechangou, Angèle N. Tchana, Paul Fewou Moundipa

Journal of Complementary and Alternative Medical Research

Aims: Terminalia superba Engl. &amp; Diels, also known as “Mbonga”, is a medicinal plant used by the “Baka” Pygmies of Cameroon for the management of male infertility. Many studies have demonstrated its anti-inflammatory, antiulcerative, antidiabetic and antioxidant properties. No scientific evidence to date has been provided on this treatment. Aluminium chloride is an endocrine disruptor that can induce male infertility through various mechanisms. The present study was designed to assess the protective effect of the bark-aqueous extract of Terminalia superba against aluminium chloride-induced reproductive toxicity in male rats. Study Design: Experimental design. Place and Duration of Study: Laboratory of Pharmacology and Toxicology, Faculty of Science, University of Yaoundé I, between November 2021 and October 2022. Methodology: Thirty-five Wistar albino rats were divided into groups of 5 animals each including untreated group. After induction of reprotoxicity by an intraperitoneal administration of aluminum chloride (20 mg/kg, body weight) for 56 days, 20 rats were subsequently orally treated for 56 days with the bark-aqueous extract at doses of 43 and 86 mg/kg as well as a co-administration of vitamin E (100 mg/kg) and zinc (50 mg/kg). The first group served as the normal control group and the last groups received only the extract (43 and 86 mg/kg). On the last day, sexual behaviour tests were performed, then rats were killed the next day. Assessment of biochemical markers of oxidative stress, histopathological studies and sperm analysis were performed. Results: Aluminium chloride induced significant decreases (P &lt;0.05) in sperm quality, sexual performance of the male rats, catalase activity and glutathione levels. The aqueous extract of Terminalia superba reversed the toxic effects of aluminium chloride by improving the sexual behaviour of male rats and increasing the level of reduced glutathione as well as the catalase activity in their testis and epididymis. Conclusion: Terminalia superba has an enhancing effect against aluminium chloride-induced toxicity in male rats, and could therefore be an alternative to solve reprotoxicity problems.

• #aluminum toxicity and tolerance in plants and animals
• #pharmacological effects of medicinal plants
• #combretaceae
• #oxidative stress

UHPLC-MS/MS profiling and in vivo aphrodisiac and androgenic effects of the aqueous extract of the roots of Schumanniophyton magnificum (K. Schum.) Harms

Patrick Kevin Moto Feune, Paulin Teko Keumedjio, Sara Nathalie Edjenguèlè Béboy, Yannick Sani Jignoua, Fabiola Jenny Massah, Sylvestre Tsila Saidou et 3 autre(s)

Journal of Ethnopharmacology

• #natural compound pharmacology studies
• #ethnobotanical and medicinal plants studies
• #herbal medicine research studies
• #aphrodisiac

Effects of the aqueous extract of Pycnanthus angolensis (Myristicaceae) on male rat sexual behavior and nitric oxide release

Sara Nathalie Edjenguèlè Béboy, Louise Marie Coûte-Chère Mbog, Yannick Sani Jignoua, Paulin Teko Keumedjio, Patrick Kevin Motto Feune, Paul Fewou Moundipa

Zenodo (CERN European Organization for Nuclear Research)

Erectile dysfunction is a major public health problem leading to harmful consequences for couples. Hence the need to find adequate treatments using medicinal plants such as <em>Pycnanthus angolensis</em>, a Cameroonian plant used to treat erectile dysfunction. The effects of aqueous extract of <em>Pycnanthus angolensis</em> wood were evaluated on male rat sexual behavior and nitric oxide release. Adult male <em>Wistar </em>rats were divided into 4 groups of 5 each that included a group receiving orally: distilled water (10 ml/Kg BW), plant extract treatment (43 or 86 or 172 mg/kg BW). Sexual behaviors were monitored on days 1, 4, 7, and 14 by pairing male rats to receptive females and nitric oxide levels were assessed in penile homogenate. At the end of the 14 days of treatment, rats were killed and sexual organs weighed. No significant effect was observed on sexual organs weights. When administered at a dose of 43 mg/Kg, body weight; the plant extract significantly increased (p&lt;0.05) the sexual performance as well as the libido of male rats. Nitric oxide release was significantly increased (p&lt;0.05) in that group of extract-treated rats, while compared to the control group. The study demonstrates that <em>Pycnanthus angolensis</em> is a medicinal plant which can enhance the sexual behaviors of male rat. Hence, it can be used for the management of erectile dysfunction, mainly at a dose of 43 mg/Kg, body weight.

• #medicinal plant extracts effects
• #nitric oxide
• #traditional medicine
• #chemistry