Dominique NGNINTEDO

2026Article de revue

Isolation, RP‐UHPLC‐ESI‐LIT‐Orbitrap‐MS/MS‐Based Metabolic Profiling and Anticandidal Activity of the Root and Leaf Secondary Metabolites of Monotes Kerstingii Gilg (Dipterocarpaceae)

Sorelle Kache Fotsing, Dominique Ngnintedo, Yanick Kevin Dongmo Melogmo, Alena Soboleva, Kevine Dongmo Jumeta, Bruno Ndjakou Lenta et 7 autre(s)

Chemistry & Biodiversity

The anticandidal activity of Monotes kerstingii leaf and root crude extracts was evaluated against five clinical Candida isolates: C. albicans, C. parapsilosis, C. krusei, C. glabrata and C. tropicalis. Extracts from both organs displayed Minimal Inhibitory Concentrations (MICs) ranging from 3.9 to 2000 µg/mL. Out of the five Candida species, the leaf hydroethanolic extract (EMKL) was the most active with MIC values of 3.9, 15.6 and 31.5 µg/mL on C. krusei, C. parapsilosis and C. albicans, respectively. The chemical investigation of these extracts led to the characterization of six previously undescribed metabolites, including a glycosylated stilbene: kerstingioside (1), three cis stilbene-coumarins: cis-kerstilbcoumarin A-C (2-4) among which two as inseparable cis/trans-mixtures, one flavanone: kerstingiiflavanone (5) and one fatty acid glycoside, monestoside B (6), alongside with 22 known compounds. The distribution of the annotated metabolites in the roots and leaves was confirmed by comprehensive RP-UHPLC-Orbitrap-MS and MS/MS analysis. In addition, all the isolated compounds were screened for their anticandidal activity. Stilbene 7 was the most active constituent, with MICs of 7.8, 15.6, and 15.6 µg/mL against C. albicans, C. krusei, and C. parapsilosis, respectively, while compounds 14 and 15 exhibited only moderate activity.

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2018Article de revueAccès ouvert

Cytotoxicity of seputhecarpan D, thonningiol and 12 other phytochemicals from African flora towards human carcinoma cells

Victor Kuete, Dominique Ngnintedo, Ghislain W. Fotso, Oğuzhan Karaosmanoğlu, Bonaventure T. Ngadjui, Félix Keumedjio et 3 autre(s)

BMC Complementary and Alternative Medicine

BACKGROUND: Despite the remarkable progress in cancer therapy in recent years, this disease still remains a serious public health concern. The use of natural products has been and continues to be one of the most effective ways to fight malignancies. The cytotoxicity of 14 compounds from African medicinal plants was evaluated in four human carcinoma cell lines and normal fibroblasts. The tested samples included: β-spinasterol (1), friedelanone (2), 16β-hydroxylupeol (3), β-amyrin acetate (4), lupeol acetate (5), sequoyitol (6), rhamnitrin (7), europetin 3-O-rhamnoside (8), thonningiol (9), glyasperin F (10), seputhecarpan B (11), seputhecarpan C (12), seputhecarpan D (13) and rheediaxanthone A (14). METHODS: The neutral red uptake (NR) assay was used to evaluate the cytotoxicity of samples; caspase-Glo assay, flow cytometry for cell cycle analysis and mitochondrial membrane potential (MMP) as well as spectrophotometry to measure levels of reactive oxygen species (ROS) were performed to detect the mode of action of compounds 9 and 13 in MCF-7 breast adenocarcinoma cells. RESULTS: values varied from 0.36 μM (against MCF7 cells) to 5.65 μM (towards colon carcinoma DLD-1 cells) for 9, from 9.78 μM (against MCF7 cells) to 67.68 μM (against HepG2 cells) for 13 and 0.18 μM (towards HepG2 cells) to 72 μM (towards Caco-2 cells) for the reference drug, doxorubicin. Compounds 9 and 13 induced cell cycle arrest in Go/G1 whilst doxorubicin induced arrest in G2/M. The two molecules (9 and 13) also induced apoptosis in MCF-7 cells through activation of caspases 3/7 and 9 as well as enhanced ROS production. CONCLUSION: Compounds 9 and 13 are good cytotoxic phytochemicals that should be explored more in future to develop a cytotoxic drug to fight human carcinoma.

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2016Article de revue

MOESM1 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

Dominique Ngnintedo, Ghislain W. Fotso, Victor Kuete, Frederic Nana, Louis P. Sandjo, OÄuzhan KaraosmanoÄlu et 5 autre(s)

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Additional file 1. Comparision of 1H and 13C NMR spectra of seputhecarpan C 1 and seputhecapan B (Fotso et al, 2013). These spectra clearly show the presence of an additional methoxyle group in seputhecarpan C.

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2016Article de revue

MOESM1 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

Dominique Ngnintedo, Ghislain W. Fotso, Victor Kuete, Frederic Nana, Louis P. Sandjo, OÄuzhan KaraosmanoÄlu et 5 autre(s)

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2015Article de revue

Three new isoflavonoids with antioxidant properties from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae)

Ghislain W. Fotso, Fozia A. Maher, Dominique Ngnintedo, Patrick Y. Ango, Deccaux G. F. W. Kapche, Bathélémy Ngameni et 4 autre(s)

Phytochemistry Letters

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2016Article de revue

MOESM2 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

Dominique Ngnintedo, Ghislain W. Fotso, Victor Kuete, Frederic Nana, Louis P. Sandjo, OÄuzhan KaraosmanoÄlu et 5 autre(s)

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Additional file 2. 1H and 13C NMR spectra of seputhecarpan D. 2.

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2016Article de revue

MOESM2 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

Dominique Ngnintedo, Ghislain W. Fotso, Victor Kuete, Frederic Nana, Louis P. Sandjo, OÄuzhan KaraosmanoÄlu et 5 autre(s)

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Additional file 2. 1H and 13C NMR spectra of seputhecarpan D. 2.

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2016Article de revue

MOESM3 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

Dominique Ngnintedo, Ghislain W. Fotso, Victor Kuete, Frederic Nana, Louis P. Sandjo, Oğuzhan Karaosmanoğlu et 5 autre(s)

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Additional file 3. 1H and 13C NMR spectra of pythylopyrone A 3 showing the signals of the additional γ,γ-dimethylallyle group in position 4 of the molecule.

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2016Article de revue

MOESM3 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

Dominique Ngnintedo, Ghislain W. Fotso, Victor Kuete, Frederic Nana, Louis P. Sandjo, Oğuzhan Karaosmanoğlu et 5 autre(s)

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Additional file 3. 1H and 13C NMR spectra of pythylopyrone A 3 showing the signals of the additional γ,γ-dimethylallyle group in position 4 of the molecule.

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2016Article de revue

MOESM4 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

Dominique Ngnintedo, Ghislain W. Fotso, Victor Kuete, Frederic Nana, Louis P. Sandjo, Oğuzhan Karaosmanoğlu et 5 autre(s)

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Additional file 4. 1H and 13C NMR spectra of mundulea lactone 4 as well as all the 2D NMR data justifying the revision of the NMR assignment of this compound as shown in Table 2.

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2016Article de revue

MOESM4 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

Dominique Ngnintedo, Ghislain W. Fotso, Victor Kuete, Frederic Nana, Louis P. Sandjo, Oğuzhan Karaosmanoğlu et 5 autre(s)

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Additional file 4. 1H and 13C NMR spectra of mundulea lactone 4 as well as all the 2D NMR data justifying the revision of the NMR assignment of this compound as shown in Table 2.

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2016Article de revueAccès ouvert

Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

Dominique Ngnintedo, Ghislain W. Fotso, Victor Kuete, Frédéric Nana, Louis P. Sandjo, Oğuzhan Karaosmanoğlu et 5 autre(s)

Chemistry Central Journal

Ptycholobium is a genus related to Tephrosia which comprises only three species. Compared to Tephrosia , which has been phytochemically and pharmacologically studied, Ptycholobium species have only few or no reports on their chemical constituents. Moreover, no studies on the cytotoxic activities of its secondary metabolites have been previously documented. From the non polar fractions of the roots bark of Ptycholobium contortum (syn Tephrosia contorta ), two new pterocarpans: seputhecarpan C 1 and seputhecarpan D 2 and a new pyrone derivative, ptycholopyrone A 3 were isolated. Alongside, five known compounds identified as 3-α,α-dimethylallyl-4-methoxy-6-styryl-α-pyrone or mundulea lactone 4 , glyasperin F 5 , seputhecarpan A 6 , seputheisoflavone 7 and 5- O -methyl-myo-inositol or sequoyitol 8 were also obtained. Their structures were established by the mean means of spectroscopic data in conjunction to those reported in literature. The NMR assignment of the major compound mundulea lactone 4 is revised in this paper. In addition, the cytotoxicity of the isolated metabolites was evaluated on two lung cancer cell lines A549 and SPC212. 8 was not active while compounds 1, 2, 4–7 displayed antiproliferative effects against the two carcinoma cell lines with IC 50 values below 75 µM. IC 50 values below 10 µM were obtained for 4, 6 and 7 on SPC212 cells. Based on the obtained results, Ptycholobium contortum turns to be a rich source of phenolic metabolites among them some bearing prenyl moieties. This study reports for the first time the isolation of pyrone derivatives 3 and 4 from Ptycholobium genus. The cytotoxicity observed for the isolate is also reported for the first time and shows that 4, 6 and 7 could be chemically explored in order to develop a hit candidate against lung cancer. Two new pterocarpans and a new pyrone derivative with cytotoxic activities from ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone.

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Dominique NGNINTEDO

Synthèse des publications

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Publications

2026

Isolation, RP‐UHPLC‐ESI‐LIT‐Orbitrap‐MS/MS‐Based Metabolic Profiling and Anticandidal Activity of the Root and Leaf Secondary Metabolites of Monotes Kerstingii Gilg (Dipterocarpaceae)

2018

Cytotoxicity of seputhecarpan D, thonningiol and 12 other phytochemicals from African flora towards human carcinoma cells

2017

Cytotoxicity of epunctanone and four other phytochemicals isolated from the medicinal plants Garcinia epunctata and Ptycholobium contortum towards multi-factorial drug resistant cancer cells

2016

MOESM1 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

MOESM1 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

2015

Three new isoflavonoids with antioxidant properties from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae)

MOESM2 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

MOESM2 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

MOESM3 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

MOESM3 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

MOESM4 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

MOESM4 of Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

Two new pterocarpans and a new pyrone derivative with cytotoxic activities from Ptycholobium contortum (N.E.Br.) Brummitt (Leguminosae): revised NMR assignment of mundulea lactone

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