Dakolé Daboy Charles

<i>Callistemon Citrinus, Cymbopogon Citratus, and Oxalis Barrelieri </i>Extracts Stimulate Defence of Tomato Against <i>Fusarium</i> Wilt

Dakolé Daboy Charles, Dongmo Lekagne Joseph Blaise, Martin Fonkoua, Julienne Nguefack, Irénée Somda

Plant

In Cameroon, tomato yields remain low due to attacks by pathogens and insects. <i>Fusarium oxysporum</i> f.sp. <i>lycopersici </i>(FOL) is a fungus responsible for <i>Fusarium </i>wilt, a disease responsible for important economic losses. To contribute to the control of this pathogen, the stimulatory effect of the tomato defence system of extracts of plants in the tomato/FOL interaction was evaluated. Tomato plants were treated with the aqueous extracts (AE) of <i>Callistemon citrinus</i> (<i>C. citrinus</i>), <i>Cymbopogon citratus</i> (<i>C. citratus</i>), and <i>Oxalis barrelieri</i> (<i>O. barrelieri</i>) at 10% (W/V). After 4 days of spraying with the extracts, the plants were inoculated with a virulent strain of <i>Fusarium oxysporum</i> f.sp. <i>lycopersici</i> (FOL) in pots experiments. Tomato roots were used to determine the contents of phenols, proteins, carbohydrates, amino acids (AA) and proline. The activities of antioxidant enzymes were evaluated: ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (GPX) and superoxide dismutase (SOD). The results showed that treatment of tomato plants with extracts and their infection with FOL induced an increase in the contents of phenols, proteins, carbohydrates, lipids, amino acids and proline in tomato roots, an increase in APX, GPX, SOD activities and a reduction in CAT activity. Our results suggest that the increase and reduction of enzymatic activities, and the increase in the synthesis of some metabolites could mitigate the oxidative damage that takes place during the expansion of the pathogen. Aqueous extracts of <i>C. citrinus</i>, <i>C. citratus</i> and <i>O. barrelieri</i> could be used as natural products to stimulate the tomato defence system against FOL.

• #plant pathogens and fungal diseases
• #apx
• #cymbopogon citratus
• #fusarium oxysporum

Characterization of Compost Made from Chicken Droppings and Fish Bones, and Its Application to Tomato Cultivation

Dakolé Daboy Charles, Okala Bouillong Noelly Manuela, Molemb Nemeté Aristide, Martin Fonkoua, Dongmo Lekagne Joseph Blaise, Julienne Nguefack

Asian Journal of Advances in Agricultural Research

The effect of compost made from chicken droppings and fish bones on soil quality and tomato production was evaluated. The soil of Bokito (Central Region-Cameroon) was amended with four variants C1, C2, C3 and C4 of compost at 5%, 10%, and 15% compost/soil (w/w). The experimental device was completely randomized en bloc with 5 repetitions for each treatment. The physicochemical, biological and biochemical parameters of compost and amended soils, as well as tomato production were evaluated.&#x0D; The highest concentrations of nitrogen, phosphorus and potassium were obtained with C3 (1.96g/Kg) and C2 (0.42 g/Kg; 0.08 g/Kg) respectively. The different compost variants had a C/N ratio between 21.88 (C1) and 30.27 (C2) and their pH was basic. They were rich in exchangeable ions (Mg2+, Ca2+, K+, Na+) and poor in heavy metals (Pb, Zn, Cu). A significant increase in the C/N ratio was noted in all the amended soils compared to the unamended soil. It varied from 12.50±0.10 (unamended soil) to 30.55±2.04 (C1 10%). Exchangeable ion concentrations (Mg2+, Ca2+, K+, Na+) were higher in amended soils. The bacterial biomass varied from 347 x 105UFC/g (C4) to 609 x105UFC/g (C2) values significantly higher than that of the unamended soil (100 x105UFC/g). The fungal biomass varied from 470 x105UFC/g (C1) to 781 x105UFC/g (C2) values significantly higher than that of the unamended soil (120 x105UFC/g). The 15% C2 component presented the highest cellulase and protease activities with values 3 and 9 times higher than the unamended soil respectively. The compost variants C2 and C1 (15%) were generally more productive with respective values of 85 and 69 fruits, the tomato amended with the chemical fertilizer having given 24 fruits.&#x0D; These results show that compost made from chicken droppings and fish bones could contribute to the development of green manure to enrich the soil, thus improving tomato production.

• #banana cultivation and research
• #phytase and its applications
• #legume nitrogen fixing symbiosis
• #compost

Characterization of Compost Made from hen Manure and Wood Ash and Its Effect on Soil Properties, Production and Nutritional Quality of Tomato (Lycopersicon esculentum Mill.)

Tchinda Fokoua Stella Laure, Dakolé Daboy Charles, Molemb Nemeté Aristide, Okala Bouillong Noelly Manuela, Martin Fonkoua, Julienne Nguefack

Asian Journal of Soil Science and Plant Nutrition

The effect of a compost based on chicken manure and wood ash on the production and nutritional quality of tomatoes was evaluated. The mixtures of the constituents of each compost were made at variable m/m proportions: 25/75 (C1); 50/50 (C2); 75/25 (C3) and 100/0 (C4). Tomato cultivation in pots was done in completely randomized random blocks with 5 repetitions, and 3 compost/soil m/m proportions (5%, 10% and 15%). The physicochemical and biochemical parameters of the compost and the amended soil, as well as the production and nutritional quality of the fruits, were evaluated. The compost showed higher pH values than the unamended soil. The C3 variant exhibited the highest pH (9.97±0.00). The C/N ratio varied from 14.78±0.67 to 15.30±0.35 values higher than that of the unamended soil (11.86±0.27); exchangeable ion concentrations were higher in the compost. The microbial biomass and the enzyme activities of the compost were significantly higher than those of the unamended soil, the greatest values were obtained with the C3 variant. pH, EC, C/N ratio, and exchangeable ion concentrations were higher in all treated soils than in soils amended with chemical fertilizer. Tomato production was higher with the variant (15% C3) with a value of 156.00 ± 1.00 fruits, the soil amended with chemical fertilizer having given 14.00 ± 1.00 fruits. Biological tomatoes obtained with compost were richer in lycopene, vitamin C, phenolic compounds and minerals than those obtained with chemical inputs (Chemical tomatoes). The compost has improved the production and nutritional quality of the tomato, which is an efficient alternative for sustainable agriculture.

• #composting and vermicomposting techniques
• #growth and nutrition in plants
• #banana cultivation and research
• #compost

Compost Made from Cow Dung and Sawdust Improves the Physicochemical and Biochemical Properties of the Soil, the Production and the Nutritional Quality of Tomatoes (Lycopersicon esculentum Mill.)

Lekini Bela Jean Gérard, Dakolé Daboy Charles, Molemb Nemeté Aristide, Okala Bouillong Noelly Manuela, Martin Fonkoua, Julienne Nguefack

Asian Journal of Soil Science and Plant Nutrition

The effect of a compost based on cow dung and sawdust on the production and nutritional quality of tomatoes was evaluated. The compost was prepared by mixing the different constituents in variable mass/mass proportions: 25/75, 50/50, 75/25 and 100/0. The experimental device for growing tomatoes in pots was made in completely randomized random blocks with 5 repetitions, and 3 compost/soil mass/mass proportions (5%, 10% and 15%) for each variant. The physicochemical, biological and biochemical parameters of the compost and amended soils, the production and the nutritional quality of the fruits were evaluated. The C3 variant of compost showed the highest pH (9.88 ± 0.01); C4 presented the highest C/N ratio (21.45 ± 0.42); C2 showed the highest contents of nitrogen, phosphorus, magnesium, calcium, potassium, and sodium, the highest values of bacterial and fungal flora and cellulase, protease, β-glucosidase, acid and alkaline phosphatase activities. Analysis of amended soils showed that: C2 (15%) has the highest pH. C1 (10%) presented the highest C/N ratio (42.12 ± 3.98) and C3 (15%) the highest contents of nitrogen, magnesium ions and calcium. C4 (15%) presented the highest potassium and sodium content. The compost significantly improved tomato production. The C2 (15%) compost was the most productive with 154 ± 3.00 fruits, 10 times more than the tomato produced with chemical fertilizer (15.00 ± 1.00 fruits). The Biological tomato showed higher proportions of ash, total sugars, lycopene, vitamin C and polyphenols than the chemical tomato. The results show that the organic treatment improved the physicochemical, biological and biochemical properties of the soil, the production and the nutritional quality of the tomato. The compost constitutes therefore an efficient method for sustainable agriculture.

• #composting and vermicomposting techniques
• #plant growth and agriculture techniques
• #agronomic practices and intercropping systems
• #compost

Differential Changes in Some Antioxidant Enzymes and Biomolecules in Leaves of Tomato Infected by Phytophthora infestans (Mont.) de Bary

Dakolé Daboy Charles, Bekono Mamdjara Larry Axel, Martin Fonkoua, Molemb Nemeté Aristide, Dongmo Lekagne Joseph Blaise, Julienne Nguefack et 1 autre(s)

Asian Journal of Advances in Agricultural Research

In Cameroon, tomato yields remain low due to attack by pathogens and insects. Phytophtora infestans (PI) is a phytopathogenic fungus responsible for the downy mildew of tomatoes, a fungal disease responsible for enormous economic losses. To contribute to the search for means of control, the stimulatory effect of the tomato defence system by extracts of some plants was evaluated. Tomato plants were treated with the aqueous macerates of Callistemon citrinus (C. citrinus) and Cymbopogon citratus (C. citratus) at 10% (W/V). After 4 days of spraying with the macerates, plants were inoculated with a virulent strain of PI. Tomato leaves collected at 2-day intervals for 10 days were used to determine the contents of chlorophyll a, proteins, carbohydrates, lipids, amino acids and proline. The activities of the main antioxidant enzymes were also assessed: Ascorbate Peroxidase (APX), Phenylalanine Ammonia Lyase (PAL), Peroxidase (POX), Superoxide Dismutase (SOD). The results showed that treatment of tomato plants with aqueous macerates of C. citrinus and C. citratus and their inoculation with PI induced an increase in chlorophyll a, protein, carbohydrate, lipid, amino acid and proline contents in tomato leaves; an increase in APX, PAL, POX, and SOD activities. Our results suggested that increased metabolite synthesis on the one hand, and increased enzymatic activities, on the other hand, could mitigate the oxidative damage that occured during pathogen expansion. Aqueous macerates of C. citrinus and C. citratus could be used as natural products to stimulate the defence system in tomato against Phytophtora infestans.

• #agriculture, plant science, crop management
• #apx
• #cymbopogon citratus
• #chemistry

Plant Extracts Induced Resistance to Fusarium oxysporum f.sp. lycopersici in Tomato

Dakolé Daboy Charles, Azah Udom René, Martin Fonkoua, Dongmo Lekagne Joseph Blaise, Julienne Nguefack, Irénée Somda

International Journal of Plant & Soil Science

Tomato (Lycopersicon esculentum Mill.) is the most consumed vegetable in the world after potato. In Cameroon, the plant is cultivated in almost all agroecological areas, however, yields remain low due to attacks by various pathogens and insects. Among the pathogens, Fusarium oxysporum f.sp. lycopersici (FOL) is a phytopathogenic fungus responsible for Fusarium wilt, a disease responsible for enormous economic losses. To contribute to the control of this microbial pathogen, the stimulatory effect of the tomato defence system of extracts of some plants in the tomato/FOL interaction was evaluated. Tomato plants were treated with the aqueous extracts (AE) of Callistemon citrinus (C. citrinus), Cymbopogon citratus (C. citratus), and Oxalis barrelieri (O. barrelieri ). After 4 days of spraying with the extracts, the plants were inoculated with a virulent strain of Fusarium oxysporum f.sp. lycopersici (FOL) under controlled conditions. Tomato roots were used to determine the contents of hydrogen peroxide (H2O2), phenols and malondialdehyde (MDA). The activities of the main antioxidant enzymes were also evaluated in tomato roots: catalase (CAT), phenylalanine ammonia lyase (PAL), peroxidase (POX) and superoxide dismutase (SOD). The results showed that treatment of tomato plants with plant extracts and their infection with FOL induced an increase in the contents of H2O2, phenols and MDA in tomato roots; an increase in PAL, POX, SOD activities and a reduction in CAT activity. Our results suggest that the increase and reduction of enzymatic activities, and the increase in the synthesis of some metabolites could mitigate the oxidative damage that takes place during the expansion of the pathogen. Aqueous extracts of C. citrinus, C. citratus and O. barrelieri at 10% (W/V) could be used as natural products to stimulate the tomato defence system against FOL. These results could contribute to the development of natural products to induce tomato resistance against FOL, thus improving productivity, quantity and quality of production.

• #plant pathogens and fungal diseases
• #essential oils and antimicrobial activity
• #plant-microbe interactions and immunity
• #fusarium oxysporum

Stimulatory Effects of Flakes and Compost Amendment Based on &amp;lt;i&amp;gt;Tithonia diversifolia&amp;lt;/i&amp;gt; on the Quality of PIF Plantain Seedlings Growth and Tolerance to &amp;lt;i&amp;gt;Mycosphaerella fijiensis&amp;lt;/i&amp;gt;

Meshuneké Arouna, Dakolé Daboy Charles, Wassom Damien Fabrice, Fotsing Silatsa Landry, E Gastón, Kengoum Djam Marie Paule et 5 autre(s)

Plantain (<i>Musa </i>AAB) in the banana family of <i>Musaceae</i>, contribute to food security for sub-Saharan African population due to its high energy value (128.6 kcal/100 g), minerals (potassium, magnesium, calcium, phosphorus), dietary fiber and vitamins (A, B and C) as well as poverty alleviation for millions of people in these regions. However, plantain production in Africa and Cameroon in particular still encounters numerous problems despite these performances. Some of these problems include the decline of soil, ineffective control methods, parasitic constraints and principally, the unavailability of quantity and quality seedlings. The use of micropropagation technique as an alternative of seedlings unavailability in quantity and quality has been explored, yet, it requires expensive laboratory equipment’s and technical skills and is not affordable by small scale farmers. Chemical control remains the principal method which consists in the use of pesticides, fungicides and herbicides that is reasonably efficient, yet the use of these chemical products shows some limits such as toxicity to the environment and human, costly to small scale farmers, pathogen resistance in plants and destruction of non-targeted species. The production of plantlets from stem bits (PIF) plantain seedlings in substrates with organic inputs like <i>Tithonia diversifolia</i> flakes and compost could be an alternative to the problem of growing healthy and good quality plantain plants. The objective of this research is to evaluate the stimulatory effects of flakes and compost amendment based on<i> Tithonia diversifolia </i>on the quality of PIF plantain seedlings growth and tolerance to <i>Mycosphaerella fijiensis </i>in nursery. The vegetative growth parameters, susceptibility to black Sigatoka disease and accumulation of biomarkers were assessed in the sterilized and non-sterilized substrate state. Flakes and compost amendment based on <i>T. diversifolia</i> significantly increase the height and the diameter of pseudo-stems, the total leaf area but also protect the seedlings against BSD up to about 89% compared to the control ones. They enhance the accumulation of biomarkers such as chlorophyll, sugars, amino acids, polyphenols, proteins, content and defense-related enzymes (peroxidase, polyphenoloxidase, phenylalanine ammonia lyase and ascorbate peroxidase). Flakes and compost amendment based on <i>T. diversifolia</i> seem to act as vital stimulators. They can therefore be seen as a tool for more sustainable and resilient agriculture, and poverty reduction for poor small farmers.

• #ecology and conservation studies
• #tithonia
• #amendment
• #compost

Effect of essential oil and powders of Tithonia diversifolia on the growth parameters and yield of rice in the field

Nanfack Albert Dongmo, Julienne Nguefack, Fouelefack François Romain, Dakolé Daboy Charles, Kuate Tueguem William Norbert, Nkengfack Augustin Ephraïm et 1 autre(s)

International Journal of Current Research in Biosciences and Plant Biology

The high costs of agricultural inputs push to look for alternatives to increase the production of rice. For this, the objective would be to evaluate in the field, the effect of seed treatment using the essential oil and the improvement of plants using Tithonia diversifolia powders on rice growth. The experiment was carried out in the Department of Moungo, in the locality of Lelem and Kompina. Before the field experiment, the biochemical parameters of the powders and the soil physicochemical were determined. The experimental design is a completely randomized factorial split-plot consisting of three treatments and two varieties (NERICA 3 and NERICA 8) with four replicates. The present study was carried out during two successive seasons of 2018 and 2019. All data are the meaning of these seasons. It appears that the soil of Kompina is an acid (pH between 5.0 and 5.3) and sandy clay that of Lelem is silty-clay-sandy and slightly basic (pH between 6.8 and 7.9). The yield increase is greater in the treated plots than control. Yield is high in the locality of Lelem than in the locality of Kompina. The 1000 seeds weights are very large in the amended plots (33 g) than the control plots (29 g). It is observed a good emergence rate (96%) in the two localities and some symptoms of the disease transmitted by seeds were observed only in the area of Kompina. From this study, we can retain that the NERICA varieties adapt very well to the rainy conditions of up-land and deserve to be popularized. The positive results of the amendment to the powder and treatment of seeds with essential oil on the growth of rice deserve a renewed interest in the exploitation of this plant resource as biofertilizer and biopesticide to improve rice production.

• #biological control of invasive species
• #medicinal plants and neuroprotection
• #aquatic life and conservation
• #tithonia

Antifungal potential and phytochemical analysis of extracts from seven Cameroonian plants against late blight pathogen Phytophthora infestans

Joseph Hubert Galani Yamdeu, Julienne Nguefack, Dakolé Daboy Charles, Petchayo Tigang Sandrine, Fouelefack François Romain, Amvam Zollo et 1 autre(s)

Laboratory of Plant Biochemistry, Department of Biochemistry, B.A. college of Agriculture, Anand Agricultural University, Anand-388110, Gujarat, India. Laboratory of Phytobiochemistry and Study of Medicinal Plants, Department of Biochemistry, Faculty of Science, University of Yaounde 1, PO. Box 812, Yaounde, Cameroon Institute of Agricultural Research for Development (IRAD), Box 2123, Yaounde, Cameroon *Corresponding author e-mail: josephgalani@gmail.com

• #seed and plant biochemistry
• #plant pathogens and resistance
• #plant pathogens and fungal diseases
• #phytophthora infestans

Cultivation of finnochio fennel.

M.I. Morales, Dakolé Daboy Charles, J. E. Simón

Europe PMC (PubMed Central)

• #plant physiology and cultivation studies
• #fungal biology and applications
• #mathematics