Publications scientifiques
Clautilde Teugwa Mofor
Liliane Nangmo Liliane Nangmo
Fabrice Fekam Boyom
Dénis Zofou
Vincent P.K. Titanji
Paul Henri Amvam-Zollo Paul Henri Amvam-Zollo
International Journal of Indigenous Herbs and Drugs
Objective: The continuous spread of drug-resistance underlies the urgent need for new drugs against this disease. Considering the serious implications of oxidative stress in the malaria pathology, the present work was prompted, aiming at investigating the antioxidant and anti-plasmodial activity of Fagara macrophylla (Rutaceae) and Eremomastax speciosa (Acanthaceae), two Cameroonian medicinal plants popularly employed in handling malaria and several degenerative diseases. Methods: The anti-plasmodial activity was assessed using the semi-automated method of Desjardins with the parasite lactate dehydrogenase assay (pLDH) as parasite quantification system, whereas the cytotoxicity was assessed on the monkey kidney epithelial cell line (LLC-MK2) using the MTT - ELISA assy. The antioxidant evaluation was done using 1,1-diphenyl-2-picrilhydrazyl (DPPH) for free radical-scavenging properties of the extracts and the Folin-Ciocalteu method in determining their phenol contents. The phytochemical screening of both extracts was equally conducted using standard methods. Results: The results revealed a wide range of anti-plasmodial activity in both plants, with the hexane extract of F. macrophylla stem bark showing the highest activity (IC50 = 4.94 μg/mL), followed by the methanol extract of E. speciosa leaf (IC50 = 8.85 μg/mL). More interestingly all the extracts with significant antimalarial activities did not exhibit any sign of cytotoxicity on monkey kidney cells even at 500 μg/mL. Extracts from both plants exhibited significant antioxidant properties as illustrated by their high phenols contents and scavenging activities. Conclusion: A diversity of chemical families was detected from these extracts, indicating that F. macrophylla and E. speciosa could serve as good starting point for the development of new phytomedicines and/or drug molecules against malaria and degenerative diseases.Keywords: , ,, ,
