Publications scientifiques
E. C. E. Aboudi
M. K. Tandjang
A. K. Tchana
B.T. Ngadjui
Khaya grandifoliola (Meliaceae) and Entada africana (Fabaceae ) are traditionally used as source of medicines against liver related d iseases. But the most efficient solvent to extract the plants bioactive compounds h as not yet been found. This work aimed at evaluating and comparing the hepatoprotect ive and antioxidant activities of Hexane (HE), methylenechloride-methanol (MCME) and water (WE) extracts of the plants stem bark. The hepatoprotective activity was evaluated by acetaminopheninduced damage in rat liver slices in measuring lac tate dehydrogenase (LDH) leakage as toxicity marker. The antioxidant activity was as sessed by using 2, 4-diphenyl-1picryl-hydrazil (DPPH), β-carotene-linoleic acid system ( β-CLAMS) and microsomal lipid peroxidation (MLP) assays. The MCME extract of both plants efficiently decreased LDH leakage from liver slices. At the tes ted concentration of 100µg/ml, the hepatoprotective percentage of the extract of K. grandifoliola (87.15%) and that of silymarine (93.73%) were comparable. In the antioxi dant study, the MCME and WE extracts of both plants performed well in inhibitin g MLP assay but, the MCME extracts were the more antioxidant with IC 50 values of 2.7±0.05µg/ml and 0.50±0.07µg/ml comparable with Trolox (2.27±0.52µg/ml) for K. grandifoliola and E. africana , respectively. Phytochemically, many classes of co mpounds including polyphenols were tested positive in the MCME extracts. From the interesting activity of these extracts, it might be concluded that methy lenechloride-methanol (1:1v/v) mixture is the most efficient solvent to extract he patoprotective and antioxidant active principles from K. grandifoliola and E. africana stem bark.
