Publications scientifiques
Zachée Ambang
P J Ngoh Dooh
G. Essono
Godwill M Chewachong
Carnot Charles Asseng
Plant Omics
Recently, a number of researchers have shown interest in finding out the potential of natural substances of plant origin in crop protection. A study was carried out in Yaounde (Cameroon) to test the antifungal activity of extracts of Thevetia peruviana seeds. Crude extracts were obtained after successive maceration with hexane, ethyl acetate and methanol. Seed extracts were tested for inhibition of P. megakarya, a causal agent of black pod disease of Theobroma cacao. Four strains of P. megakarya (BOK 11; BOYO; TA121 and TA123) were used. Antifungal tests were performed by using three concentrations (12.5, 25 and 50 l ml-1) of crude extracts. Seven days after incubation on pea agar medium, the results showed that the use of 50, 25 and 12.5 l.ml-1 of crude extracts with ethyl acetate resulted in 100, 70 and 50% inhibition rates respectively on P. megakarya. Methanol extract on the BOYO, TA121 and TA123 strains led to total inhibition, while 37.5, 43.2 and 63.9 % inhibition rates were recorded for BOK11 strain at 12.5, 25 and 50 l.ml-1 concentrations of the extract respectively. These results suggest that crude extracts from T. peruviana seeds are efficient biocide substances with antifungal activity.
