Publications scientifiques
Edwige Ymele Chiogo Vouffo
Autres affiliations : United States Food and Drug Administration · Center for Drug Evaluation and Research
Roméo Joël Temdié Guemmogne* (Auteur correspondant)
Borris Rosnay Galani Tietcheu
Josee Bianzoumbe
Bertrand Dabole Ladane
Autres affiliations : University of Ngaoundéré
Marc Germain Kuum Minoue
Fidèle Ntchapda
Autres affiliations : University of Ngaoundéré
Current Therapeutic Research
Acetaminophen-induced liver injury remains a significant public health problem, as available treatments are limited due to their adverse effects. Medicinal plants, which are an important source of bioactive molecules, could be an alternative treatment for liver disease. This study was designed to investigate the curative effect of aqueous extracts of Cissus quadrangularis (Vitaceae) and Jatropha gossypiifolia (Euphorbiaceae) on acetaminophen-induced liver injury in mice. Mice were divided into groups and treated with distilled water, silymarin (50 mg/kg), a reference hepatoprotective agent, and aqueous extracts of C. quadrangularis and J. gossypiifolia (50 and 100 mg/kg, p.o. respectively). These substances were given as a single daily dose two hours after acetaminophen (APAP) administration (300 mg/kg, p.o.) for two days. Mice were sacrificed 24 hours after the last dose and serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, total bilirubin and protein levels, reduced glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and nitrite tissue levels were assessed. Histology of the livers of the mice was performed by hematoxylin/eosin staining. APAP administration induced a significant (P < 0.05) body weight loss (-14.45±5.92%), a significant elevation of ALT activity (15.08%), total protein and bilirubin levels (25.80%), and a significant (P < 0.05) increase in liver SOD (67.71%), CAT (63.00%), GSH (40.29), MDA (30.67%), and nitrite levels compared to the control group. In curative treatment, C. quadrangularis and J. gossypiifolia (50 and 100 mg/kg) significantly (P < 0.05) reduced body weight loss (16.67±7.16 and 1.25±0.51%, respectively), serum ALT activity (17.62 and 11.14%, respectively), bilirubin level (29.62 and 49.14%, respectively) compared to APAP group, and J. gossypiifolia normalised serum total protein level. Both extracts significantly (P < 0.05) reduced the levels of GSH and MDA and normalized that of nitrite, SOD, CAT, compared to the APAP group. Hepatocyte necrosis and inflammatory cell infiltration were remarkably reduced by the plant extracts. The results obtained are evidence in favor of the development of a formulation based on the extracts of these plants against liver diseases.
