Publications scientifiques
2019AutreanglaisAccès ouvert
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Publications scientifiques
Abdel Jélil Njouendou
Chi Anizette Kien
Mathias Eyong Esum
Winston Patrick Chounna Ndongmo
Fanny Fri Fombad
Narcisse Victor Tchamatchoua Gandjui
Peter Enyong
Samuel Wanji* (Auteur correspondant)
Manuel Ritter
Kenneth Pfarr
Autres affiliations : German Center for Infection Research
Laura E. Layland
Autres affiliations : German Center for Infection Research
Achim Hoerauf
Autres affiliations : German Center for Infection Research
Joseph D. Turner
Data in Brief
Endemic communities of Mansonella perstans infections have been neglected since associated pathology remains undefined. Consequently, improvements in drug therapy have also been ignored despite a large number of infected individuals in areas of Cameroon. Thus, we established an in vitro system to culture M. perstans microfilariae (Mf); the transmission stage of infection. In short, we compared the ability of two renowned culture media (Dulbecco's Modified Eagle's Medium (DMEM) and Roswell Park Memorial Institute (RPMI-1640)) to sustain Mf in culture. Media were supplemented with 10% fetal bovine serum (FBS) and monkey kidney epithelial cells (LLC-MK2) were used as feeder cells. As readout we assessed Mf survival and motility using a standardised microscopy assessment strategy. Moreover, this in vitro culture system was used to test susceptibility levels of microfilariae to different chemotherapeutic agents. Parasite motility was scored daily using a graded system and analysed using the average motility and area under the motility curve of M. perstans Mf. These datasets were analysed and discussed in detail in the related article entitled: “In vitro maintenance of Mansonella perstans microfilariae and its relevance for drug screening” [1].