Publications scientifiques
2019Article de revueanglais
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Publications scientifiques
Andrea Rosanoff
Pierre S. Haddad
Léonard Tedong
PD Chuisseu
SD Sockeng FM Kanmangne
Desire Paul D Dzeufiet
International Journal of Complementary & Alternative Medicine
Cashew, Anacardium occidentale Linn of the family Anacardiaceae, has been documented as traditional plant treatment for diabetes and hypertension.2][3] We then wondered what molecular compounds were involved in these potent anti-diabetic activities.In the present study, using antioxidant activity-guided fractionation (DPPH advanced glycation endproducts (AGEs) essays), four extracts (E1 to E4) of A. occidentale leaves were studied for possible antioxidant capacities and vascular activity.We also compared the protective effects of cashew leaf extracts against high glucose mediated toxicity in cultured bovine aortic endothelial cells (EAhy 926 cells).Total antioxidant activity of the extracts of A. occidentale and identified compounds was determined using DPPH free radical scavenging assay.Two extracts of cashew's leaves, E3 and E4, were found to possess significant DPPH radical-scavenging activity with equivalent trolox values of 218±8 and 760±3 mg trolox/g dried extract, respectively.The order of the radical scavenging potency is E4>E3>E2>E1.In vitro AGEs generation after 24h revealed that E3 had the strongest inhibitory activity against AGEs formation and its IC 50 value was 0.070mg/ml compared to that of aminoguanidine as standard (0.826mg/ml).A significant (p<0.05)increase in fluorescence of AGEs in 25.0mM glucose (HG) was partially corrected by E3, E4 and agathisflavone (AF) (p<0.05) vs. 25mM glucose alone.Additionally, cotreatment with E3, E4 or AF significantly attenuated HG induced EAhy as judged by overexpression of Mn-SOD and NF-kB.These results suggest that A. occidentale MetOH extracts together with its principal compound, identified as agathisflavone reduces high glucose-induced toxicity by attenuating AGEs, MnSOD, NF-kB generation in endothelial cells, and therefore may be of significant therapeutic benefits against oxidative stress in diabetic complications and hypertension.