Publications scientifiques
Brice Enang
Sylvain Nsangou Pechangou
Marie Louise Medoua
Zenodo (CERN European Organization for Nuclear Research)
<strong>Background</strong>: Wild edible fruits are good source of phenolic compounds such as anthocyanins. Those compounds are known for their anti-inflammatory potential. They interact with many mediators of the physiopathological pathway such as NF-kB which is at a central position in the inflammatory response in macrophages. This study aimed to determine the anti-inflammatory and antioxidant potential of ten wild edible fruits aqueous extracts. <strong>Methods: </strong>The antioxidant potential was evaluate through the DPPH, ABTS and OH radical scavenging activity; the ferric reducing agent power and the total antioxidant capacity assessment. Also, the total phenolic and flavonoids, anthocyanins compounds and vitamin C content were assayed. For the anti-inflammatory activity, a primary macrophage culture was used. Inflammatory mediators such as nitric oxide (NO), alkaline phosphatase, NADPH oxidase and 5-lipoxigenase activity was assayed. <strong>Results: </strong><em>Alchornea cordifolia</em> and <em>Pentaclethra macrophylla </em>extracts showed the greatest antioxidant activity respectively (Antiradical power (AP)=5.82 mol of DPPH/mg of extract and AP=5.05 mol of DPPH/mg of extract for DPPH scavenging assay); (IC<sub>50</sub>=1.62µg/ml and IC<sub>50</sub>=1.60µg/ml for ABTS radical scavenging assay); (IC<sub>50</sub>=32.67µg/ml and IC<sub>50</sub>=70.01µg/ml for OH radical scavenging assay) and (EC<sub>50</sub>=54.06µg/ml and EC<sub>50</sub>=17.46µg/ml for ferric reducing antioxidant power) with a non-significant difference with the reference compound. For the anti-inflammatory activity, firstly it has been observed that <em>Alchornea cordifolia’s </em>extract exhibits the greatest potential against NADPH oxidase activity inhibition (IC<sub>50</sub>=36.20µg/ml); alkaline phosphatase activation (EC<sub>50</sub>=0.27µg/ml) with non-significant difference with the reference compound. Secondly, <em>Pentaclethra macrophylla’s</em> extract demonstrated a high ability to inhibit NO production (IC<sub>50</sub>=5.79µg/ml) and 5-lipoxigenase (IC<sub>50</sub>=1.41µg/ml). <strong>Conclusion: </strong>From the results obtained the assessed wild fruit extracts appears to be good sources for anti-inflammatory and antioxidant bioactive molecules. They could be beneficial for the research of new modulators for the management of inflammatory disorders.
