Scientific publications
Yves Oscar
D. Nganso
Elisabeth M. Zeuko
Fabrice Fekam Boyom
The aim of this study was to evaluate the anticandidal activity of fruit, root, stem bark extracts and compounds from Jacaranda mimosifolia and Kigelia africana (Bignoniaceae) on four Candida albicans strains ATCCL26, ATCC12C, ATCCP37039, and ATCCP37037. The extracts from different plants part were prepared using Methanol and Methanol/Methylene Chloride. Column chromatography was used for the fractionation and isolation of compounds. Agar disc diffusion method was used to screen anticandidal activity of the extracts and compounds. Micro dilution method was used to determine the Minimum Inhibitory Concentration of the selected extracts/compounds. The compounds Benzoic acid 1, 5-carboxy-1,2,3,4,4a,7,8,8a-octahydro-1,2,4a-trimethyl-, [1S-(1α,2β,4aβ,8aα)] 2, betulinic acid 3, and ursolic acid 5 were obtained from Jacaranda mimosifolia. Also, 2-(4-hydroxyphenyl) ethyl ester 11, oleanolic acid 14, β-friedelinol 15 and pomolic acid 16 were obtained from Kigelia africana. The inhibition zone diameters varied from 0 to 10 mm on the four tested C. albicans strains. The Minimum Inhibitory Concentration ranged from 2.045 to ≥ 8.3 mg/mL. The compounds showed better activity than the extracts. The results of this study suggest that the medicinal plants represent an untapped source of compounds with anti-C. albicans activity that could be a resource in the development of new therapeutic natural products.
