Scientific publications
2025Journal articleEnglishOpen access
Checking session…
Scientific publications
FD NKOLO TOLO
JR NKECK
LN ATANWO DONGMO
Manuela Eko
N TANETCHOP MAGOUO
MJ OBONO EKAMENA
Cameroon Journal of Biological and Biochemical Sciences
A detailed understanding of the kinetics of mandibular bone consolidation requires robust experimental models. This study assesses the robustness and reproducibility of a Wistar rat model of mandibular bone lesions. The study was conducted on 24 Wistar rats, divided into three experimental groups of 08 rats. The first group served as control. The second group underwent extraction of the lower central incisor with injury to the underlying alveolar bone. The third group underwent drill holes in the mandibular symphysis. The rats were sedated by intraperitoneal injection of ketamine and diazepam. Tooth extraction was performed with an incisor forceps, and drill holes with a micromotor and steel drill, under saline irrigation. All animals received post-operative antibiotic and analgesic treatment. Alkaline phosphatase (ALP) activity was significantly increased in females (289.33 to 437 IU/L, p<0.01) and males (56.75 to 303.67 IU/L, p<0.01), indicating a more intense and prolonged osteogenic response with drilling than with tooth extraction. In females, the drill hole significantly decreased calcium to 55.5 mg/L (p<0.05), whereas in males, tooth extraction significantly increased it to 24.3 mg/L (p<0.05) and drilling to 128.25 mg/L (p<0.05), suggesting a variable bone remodeling response according to sex and lesion type. In females, phosphorus increased significantly from week 1 in the drill hole group (p<0.05), reaching a mean value of around 100 mg/L, while in males, the progressive increase in phosphorus in the same group occurred between weeks 2 and 6 (p<0.05), reaching a peak of around 120 mg/L at week 6. The results obtained, in particular the kinetics of biochemical markers, confirmed the relevance of this model for assessing the mechanisms of bone consolidation. The prospects for future research are vast and promising, paving the way for significant advances in maxillofacial surgery and regenerative medicine. Keywords: Bone Remodeling, Robustness, Reproducibility, Mouse Model, Mandibular Fracture