Scientific publications
2016Journal articleEnglish
Steroidal saponins from Chlorophytum deistelianum (opens in a new tab)
Authors
University of Yaoundé I
BT Ngadjui
Université de Bourgogne
Turibio Kuiate Tabopda
AC Mitaine-Offer
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Scientific publications
BT Ngadjui
Turibio Kuiate Tabopda
AC Mitaine-Offer
MA Lacaille-Dubois
Thomas Paululat
Stéphanie Delemasure
Patrick Dutartre
Planta Medica
Plants of the genus Chlorophytum are known as a rich source of steroidal saponins[1 – 3] which have attracted the attention for their structural diversity and significant bioactivities such as cytotoxic, immunomodulating, antifungal, insecticidal activities, just to mention a few [4]. In our continuing search for bioactive saponins from the genus Chlorophytum [1 – 3] we investigated Chlorophytum deistelianum Engl. & Krause (= C. sparsiflorum Backer var. sparsiflorum ) from a phytopharmacological point of view. C. deistelianum is a fleshy herb to 50 cm high, with white or greenish flowers. No study on the secondary metabolites of this plant has been reported so far. Therefore, we report herein the isolation of four previously undescribed steroidal saponins, called chlorodeistelianosides A – D ( 1 - 4 ) together with five known steroidal saponins from C. desteilianum . Their structures were determined by spectroscopic methods including 1D and 2D NMR experiments, ESI and HRESIMS. Compounds 1 and 2 are glycosides of hecogenin and its 24β-OH derivative, respectively, whereas 3 is a glycoside of (25 R )-5α-22α-methoxyfurostane-2α,3β,26 triol and 4 a glycoside of (25 R )-5α-furost-20(22)-en-12-one-3β,26 diol. Compounds 2 – 4 share the same sugar sequence at C-3 characterized as β-D-glucopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranosyl whereas the sugar sequence of 1 was elucidated as β-D-glucopyranosyl - (1→3)-[α-L-rhamnopyranosyl-(1→4)]-β-D-xylopyranosyl-(1→3)-[β-D-glucopyranosyl-(1→2)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranosyl. Furthermore, seven compounds were examined for cytotoxicity against one human colorectal adenocarcinoma cell line (SW480) and one rat cardiomyoblast cell line (H9c2). Among them, three known spirostane-type glycosides exhibited cytotoxicity on both cell lines with IC 50 ranging from 8 to 10µM.