Scientific publications
Jean Jules Nana Ndangang* (Corresponding author)
Christelle Kebassa Nkwefuth
Ives Magloire Kengne Noumsi
Alain-Martial Sontsa-Donhoung
Dumitra Răducanu
Narcis Bârsan* (Corresponding author)
Emilian Moșneguțu
Valentin Nedeff
Florin Nedeff
Mirela Panainte-Lehăduș
Dana Chițimuș
Anne Ayo
Guy Valérie Djumyom Wafo
Environments
This study centred on isolating and characterizing Bacillus amyloliquefaciens strains derived from wastewater sludge to assess their potential for sludge treatment. Samples were collected from the Etoa wastewater sludge treatment plant in Yaounde, Cameroon. The isolates were obtained on nutrient agar medium and were identified through morphological and biochemical characterization, followed by 16S rRNA gene sequencing analysis. The sequences showed 99–100% similarity with Bacillus amyloliquefaciens strains in the NCBI database. The isolates exhibited significant in vitro enzymatic activities, including catalase, amylase, and protease production, indicating their ability to degrade hydrogen peroxide starch and proteins, respectively. The results confirmed the in vitro potential of Bacillus amyloliquefaciens as a promising microbial agent for organic matter degradation in wastewater sludge. Although the findings were limited to laboratory conditions, they provided a foundation for future pilot-scale or in situ studies aimed at validating their practical efficiency. This research contributes to the development of microbial-based and eco-efficient strategies for sustainable sludge management.
