Scientific publications
Ridel Mbiandou Njami
Ferdinand Kouoh Elombo
Sylvain Nsangou Pechangou
Paul Fewou Moundipa
Bradley W. Bolling
Journal of Drug Delivery and Therapeutics
Entada africana Polyphenolic Extract (PEE) revealed higher contents of total polyphenols (264.91±1.51mg EAG/g of extract), flavonoids (14.26 ±0.26mg EQ.g of extract) and flavonols (2.1±0.03mg EQ/g of extract). Concentration of Scavenging 50% (CS50) of hydroxyl radical (HOo) radicals with PEE (20.65 ± 0.35 μg/mL) compared to that of vitamin C (7.90 ± 0.00 μg/mL) showed a significant difference at p< 0.05 for HOo scavenging activities. The PEE extract inhibited lipidic peroxidation with IC50 of 9.70 ± 0.21 μg/mL but not significantly different from vitamin C (8.70 ± 0.14 µg/mL). PEE extract have inhibited the production of TNFα with IC50 (71.05 ± 1.20 µg/mL) significantly (p< 0.05) when compared to 25% of methylene chloride fraction of Khaya grandifoliola (32.10 ± 1.13 µg/mL) considered as a standard. For the activation of erythrocytes plasma membrane redox system (PMRS), PEE had an Effective Concentration 50% (EC50) of 8.4 ± 0.00 µg/mL relatively less than that of quercetine 4.6 ± 0.00 µg/mL. Moreover, PEE inhibits proteins oxidation in a dose-concentration manner with an IC50 of 14.60 ± 1.13 µg/mL significantly different (p< 0.05) from that of quercetin (4.60 ± 0.00 µg/mL) considered as a standard. Altogether, PEE could be used as potential antioxidant and anti-Tumor Necrosis Factor alpha (TNFα) ingredient for food and nutrition. Keywords: Entada africana, polyphenols, antioxidant, anti-TNFα, erythrocytes PMRS
