Scientific publications
Line Made Simo
Lin Marcellin Messi
Other affiliations: Institut de Recherche Agricole pour le Développement
Fabrice Fekam Boyom
Dieudonné Emmanuel Pegnyemb
Olivier Placide Noté* (Corresponding author)
Christian D. Muller
Other affiliations: Laboratoire de Biophotonique et Pharmacologie · Université de Strasbourg
Aime-Didier Boyogueno Begoude
Mohamed Haddad
Other affiliations: Université Fédérale de Toulouse Midi-Pyrénées · Institut de Recherche pour le Développement
Planta Medica
. Their structures were established based on extensive analysis of 1D and 2D NMR data, HR-ESI-MS analysis, and by chemical degradation. The proapoptotic effect of zygiaoside E (1: ) was evaluated on human malignant melanoma (A375), human epidermoid cancer (A431), and normal Homo sapiens skin tissue (TE 353.SK.) cell lines by cytometric analysis. Zygiaoside E (1: ) induced apoptosis of the two human cancer cell lines (A375 and A431) in a dose-dependent manner at 1 µM but did not induce apoptosis in noncancerous skin cells (TE 353.Sk), even when treated with concentrations up to 15 µM. The underlying mechanism of the apoptosis induction activity of zygiaoside E (1: ) on the mitochondrial membrane potential status in A375 cells was further assessed by monitoring the uptake rate of DiOC6, a mitochondrial specific and voltage-dependent fluorescent dye. The number of malignant melanoma cells emitting high fluorescence levels was decreased when cells were treated with 3 or 5 µM of zygiaoside E (1: ) during either 12 or 24 h, thereby revealing a drop of mitochondrial membrane potential in A375 cells upon treatment, which indicated mitochondrial perturbation.
