Scientific publications
Mariscal Brice Tchatat Tali
Other affiliations: Ministry of Scientific Research and Innovation
Paul Etoga Mbarga
Darline Dize
Lauve Rachel Tchokouaha Yamthé
Other affiliations: Ministry of Scientific Research and Innovation · Institut de Recherches Médicales et d’Etudes des Plantes Médicinales
Fabrice Fekam Boyom
Other affiliations: Ministry of Scientific Research and Innovation
Mahamane Idi Issa Abdoulahi* (Corresponding author)
Ilagouma Amadou Tidjani
Hama Hamadou Habibou
Bakasso Sahabi
Michael Hermann Kengne Kamdem
Derek Tantoh Ndinteh
Chemistry & Biodiversity
ABSTRACT Malaria is a significant concern due to the emergence of artemisinin‐resistant parasites. This study aims to unveil the selective inhibitory potential of extracts and derivatives of some Niger's plants against Plasmodium falciparum . Plant extracts were screened on chloroquine‐sensitive ( Pf 3D7) and multidrug‐resistant ( Pf Dd2) P. falciparum strains using the SYBR Green method, followed by bio‐guided fractionation of active extracts. Bio‐guided method was used for the isolation. Resazurin‐based and hemoglobin quantification assays were used to assess the selectivity on normal RAW cells and erythrocytes, respectively. Among the 27 extracts, five exhibited pronounced activity (IC 50 < 5 µg/mL) against multidrug‐resistant ( Pf Dd2) and three against sensitive ( Pf 3D7) strains of P. falciparum . The methanolic extract from Phyllanthus pentandrus displayed the most promising activity the most active on both strains (IC 50 Pf Dd2 = 1.85 µg/mL and IC 50 Pf 3D7 = 4.25 µg/mL). Its fractionation led to five fractions, among which the ethyl acetate fraction was the most active (IC 50 Pf Dd2 = 3.10 µg/mL). Four compounds were isolated from the ethyl acetate fraction: kaempferol, quercetin, gallic acid, and quercetin‐3‐O‐rutinoside. Active extracts and fractions showed good selectivity on normal RAW cells and erythrocytes. This study validates the medicinal use of Niger ethnomedicine for the treatment of malaria.
